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Niese_UG_Thesis.pdf (4.61 MB)

ETD Abstract Container

Abstract Header

Fabrication of microfluidic devices to probe cell mechanical properties of MDA-MB-231 human breast cancer cells.

Niese, Brandon A
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1556626033175996

Abstract Details

2019, Bachelor of Science (BS), Ohio University, Engineering Physics.
In this thesis, I probed mechanical properties of MDA-MB-231 human breast cancer cells. To do this, I optimized the fabrication process of microfluidic devices using soft lithography methods. The flowrate inside these devices was found to be linear with respect to change in pressure. The same procedure was then used on the MDA-MB-231 cells to fi nd entry time data. Using entry time data and change in pressure data, power law rheology was used to calculate the cell fluidity of the breast cancer cells. Cell fluidity was found to be 0.21+/-0.02, which is well inside the realm of reasonable values for fluidity. Using this value and the intercept of the log-log plot of the power law rheology, the elastic modulus of MDA-MB-231 was calculated to be 278+/-6 Pa. This also agrees with most values of the elastic modulus found in the literature. These microfluidic devices in tandem with power law rheology have proven to be an effective way of calculating both cell fluidity and elastic modulus.
David Tees, PhD (Advisor)
56 p.
Biophysics; Physics
microfluidic devices; power law rheology; cancer; biophysics

Recommended Citations

Citations

  • Niese, B. A. (2019). Fabrication of microfluidic devices to probe cell mechanical properties of MDA-MB-231 human breast cancer cells. [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1556626033175996

    APA Style (7th edition)

  • Niese, Brandon. Fabrication of microfluidic devices to probe cell mechanical properties of MDA-MB-231 human breast cancer cells. 2019. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1556626033175996.

    MLA Style (8th edition)

  • Niese, Brandon. "Fabrication of microfluidic devices to probe cell mechanical properties of MDA-MB-231 human breast cancer cells." Undergraduate thesis, Ohio University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1556626033175996

    Chicago Manual of Style (17th edition)

ouhonors1556626033175996
289
© 2019, all rights reserved.
This open access ETD is published by Ohio University Honors Tutorial College and OhioLINK.