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Final Thesis - Hailey Payne.pdf (2.96 MB)

ETD Abstract Container

Abstract Header

Development of a Skin Explant Model for Studying UV-induced DNA damage and carcinogenesis

Payne, Hailey
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682075898677468

Abstract Details

2023, Bachelor of Science (BS), Ohio University, Chemistry.
There is a need for a skin model which combines the natural physiology of skin and uses less mice. Natural physiology is obtained using fresh, intact skin explants sourced from living organisms such as humans or mice. This work focused on the standardization and characterization of an in vitro mouse skin explant model for studying ultraviolet (UV) damage and repair mechanisms in skin. In this study, we established a protocol to use skin explants from discarded mice after euthanasia; these skin explants are cuts of intact dermal and epidermal skin suspended in cell culture medium and maintained in vitro. Our tissue explants maintain in vivo skin’s characteristics and physiological responses for short periods of incubation time, but use less mice. In addition, our model allows us to study DNA damage and repair and still analyze different incubation periods post-sUV.
Lauren McMills, Dr. (Advisor)
Verónica Bahamondes Lorca, Dr. (Advisor)
Shiyong Wu, Dr. (Advisor)
55 p.
Biochemistry; Chemistry
Skin; UV; DNA Damage; Biochemistry; Explant Model

Recommended Citations

Citations

  • Payne, H. (2023). Development of a Skin Explant Model for Studying UV-induced DNA damage and carcinogenesis [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682075898677468

    APA Style (7th edition)

  • Payne, Hailey. Development of a Skin Explant Model for Studying UV-induced DNA damage and carcinogenesis . 2023. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682075898677468.

    MLA Style (8th edition)

  • Payne, Hailey. "Development of a Skin Explant Model for Studying UV-induced DNA damage and carcinogenesis ." Undergraduate thesis, Ohio University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682075898677468

    Chicago Manual of Style (17th edition)

ouhonors1682075898677468
53
© 2023, all rights reserved.
This open access ETD is published by Ohio University Honors Tutorial College and OhioLINK.