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Induction of the Lipid Regulator PPAR-Delta in FoxO1 Overexpressed Skeletal Muscle

Markovic, Vesna
http://orcid.org/0000-0002-4449-8723
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525456674210299

Abstract Details

2018, Master of Science, University of Toledo, Exercise Science.
The Forkhead box protein O1 (FoxO1) molecule plays a vital role in the production of fatty acid metabolism.1,2 However, the precise mechanisms for its upregulation are yet to be fully understood.1,2 Preliminary data has shown that overexpression of FoxO1 in skeletal muscle results in a dramatic decrease in intramuscular triglyceride storage.3,4 A potent stimulator of fatty acid metabolism in skeletal muscle is peroxisome proliferator activated receptor delta (PPAR-Delta). As such, the purpose of the present study was to investigate the interplay between FoxO1 and PPAR-Delta in the promotion of fatty acid metabolism in skeletal muscle. Our hypothesis is that PPAR-Delta will have a greater protein expression in skeletal muscle overexpressing FoxO1 versus normal skeletal muscle. In this study, western blotting was used to detect PPAR-Delta expression while densitometry was used to quantify expression levels. Our findings show that PPAR-Delta expressed higher levels of protein expression in the overexpressed FoxO1 skeletal muscle versus the wildtype skeletal muscle. This suggests that PPAR-Delta may contribute to adaptive changes in fatty acid metabolism in response to FoxO1 overexpressed skeletal muscle. Further studies need to be conducted to investigate the more in-depth relationship between the interplay of PPAR-Delta and FoxO1 in fatty acid metabolism in skeletal muscle.
Thomas J. McLoughlin (Committee Chair)
Francis X. Pizza (Committee Member)
Abraham D. Lee (Committee Member)
31 p.
Biology; Physiology
PPAR-Delta; FoxO1; fatty acid metabolism

Recommended Citations

Citations

  • Markovic, V. (2018). Induction of the Lipid Regulator PPAR-Delta in FoxO1 Overexpressed Skeletal Muscle [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525456674210299

    APA Style (7th edition)

  • Markovic, Vesna . Induction of the Lipid Regulator PPAR-Delta in FoxO1 Overexpressed Skeletal Muscle. 2018. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525456674210299.

    MLA Style (8th edition)

  • Markovic, Vesna . "Induction of the Lipid Regulator PPAR-Delta in FoxO1 Overexpressed Skeletal Muscle." Master's thesis, University of Toledo, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525456674210299

    Chicago Manual of Style (17th edition)

toledo1525456674210299
332
© 2018, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.