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Non-Invasive Biomarkers of Eosinophilic Esophagitis: Blood Eosinophil Level, Eosinophil-Derived Neurotoxin, and Eotaxin-3

Konikoff, Michael R.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148043525

Abstract Details

2006, MS, University of Cincinnati, Medicine : Epidemiology (Environmental Health).
Eosinophilic esophagitis (EE) is an increasingly recognized disorder characterized by eosinophilic inflammation of the esophageal mucosa, and typically requires serial, invasive endoscopic biopsies to document the characteristic histologic features of the disorder. The aim of this study was to identify non-invasive biomarkers that correlated with disease activity and response to treatment as measured by esophageal eosinophilia. METHODS: A prospective, cross-sectional analysis was performed on 47 pediatric patients undergoing endoscopic evaluation of possible EE. Blood samples were collected for measurement of peripheral blood absolute eosinophil count (AEC) and levels of eosinophil-derived neurotoxin (EDN), eotaxin-1, -2, and -3, and interleukin-5. Stool samples were collected for measurement of EDN. Biomarker levels were correlated with esophageal eosinophil density, and differences in biomarker levels based on disease activity and treatment were determined. RESULTS: AEC, plasma EDN, and eotaxin-3 levels significantly correlated with esophageal eosinophil density (AEC r=0.56, P<0.0001; EDN r=0.54, P<0.0001; eotaxin-3 r=0.32, P=0.04), and were increased in patients with active EE versus controls (AEC 440 vs 140 eosinophils/µL, P<0.05; EDN 50.3 vs 31.1 ng/mL, P<0.01; eotaxin-3 37.7 vs 11.5 pg/mL, P<0.01). Cutoffs were established to maximize the sensitivity, specificity, and predictive values of these biomarkers alone and in combination. Eotaxin-1 and -2, interleukin-5, and fecal EDN levels did not correlate with esophageal eosinophil density, and were not increased in active EE versus controls or those with inactive EE. CONCLUSIONS: These data demonstrate that blood levels of AEC, EDN, and eotaxin-3 may have value as non-invasive biomarkers for monitoring EE.
Dr. James Heubi (Advisor)
34 p.
eosinophilic esophagitis; biomarker; eosinophil; eosinophil-derived neurotoxin; eotaxin-3

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Citations

  • Konikoff, M. R. (2006). Non-Invasive Biomarkers of Eosinophilic Esophagitis: Blood Eosinophil Level, Eosinophil-Derived Neurotoxin, and Eotaxin-3 [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148043525

    APA Style (7th edition)

  • Konikoff, Michael. Non-Invasive Biomarkers of Eosinophilic Esophagitis: Blood Eosinophil Level, Eosinophil-Derived Neurotoxin, and Eotaxin-3. 2006. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148043525.

    MLA Style (8th edition)

  • Konikoff, Michael. "Non-Invasive Biomarkers of Eosinophilic Esophagitis: Blood Eosinophil Level, Eosinophil-Derived Neurotoxin, and Eotaxin-3." Master's thesis, University of Cincinnati, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148043525

    Chicago Manual of Style (17th edition)

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© 2006, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.