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The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells

Cashdollar, Jennifer Leigh
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1163713402

Abstract Details

2006, MS, University of Cincinnati, Arts and Sciences : Biological Sciences.
RNA interference (RNAi) is a cellular process whereby gene expression is controlled via microRNA (miRNA) or small-interfering RNA (siRNA) recognition and cleavage of target messenger RNA (mRNA). RNAi also functions in the inhibition of viral propagation in a host cell, thus providing the cell a primitive immune system. The purpose of this study was to examine the mRNA levels of dicer, the gene which encodes for the Dicer protein, an RNAi component, in response to natural viral infection. Poliovirus and astrovirus were used to infect Caco-2 cells to determine the effect that each has on the expression of dicer mRNA. Using real-time RT-PCR, dicer expression was measured over the time course of eight hours of infection. It was determined that poliovirus caused dicer expression to be upregulated over one and a half fold by eight hours of infection. Astrovirus caused dicer expression to be downregulated over one-half fold by eight hours of infection. Both results were statistically significant, indicating that poliovirus and astrovirus induce changes in dicer mRNA expression levels during natural viral infection.
Dr. Stephen Keller (Advisor)
70 p.
RNA interference (RNAi); dicer; poliovirus; astrovirus; mRNA regulation; real-time PCR

Recommended Citations

Citations

  • Cashdollar, J. L. (2006). The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1163713402

    APA Style (7th edition)

  • Cashdollar, Jennifer. The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells. 2006. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1163713402.

    MLA Style (8th edition)

  • Cashdollar, Jennifer. "The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells." Master's thesis, University of Cincinnati, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1163713402

    Chicago Manual of Style (17th edition)

ucin1163713402
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© 2006, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.