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THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS

MA, CI, Miss
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1185995222

Abstract Details

2007, MS, University of Cincinnati, Medicine : Toxicology (Environmental Health).
BACH1 (BTB and CNC homolog 1) is a transcription factor that belongs to the CNC (cap'n'collar)-type bZip (basic region leucine zipper) transcription factor family. A few BACH1 target genes have been identified such as antioxidant enzyme HMOX1 (heme oxygenase 1) and NQO1 (NADPH quinone oxidoreductase 1) in redox regulation, as well as beta-globin during erythoid differentiation. Interestingly, BACH1 is rapidly induced during differentiation of mouse 3T3-L1 pre-adipocyte cells and overexpression of BACH1 in transgenic mice causes significant thrombocytopenia. Furthermore, a human study showed BACH1 is overexpressed in fetal Down syndrome brain. All these studies suggest BACH1 plays an important role in cell differentiation and organogenesis. In order to test this hypothesis, the present study is directed at setting up a feasible model by stably transfecting mouse Bach1 into human HaCaT keratinocytes. Both real-time PCR and western blotting were applied to verify BACH1 expression level in these stably transfected cell lines.
Dr. Alvaro Puga (Advisor)
27 p.
Health Sciences, Toxicology
BACH1; HMOX1; NQO1; transcription factor; stably-transfection; real-time PCR; western blotting

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Citations

  • MA, Miss, C. (2007). THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1185995222

    APA Style (7th edition)

  • MA, Miss, CI. THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS. 2007. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1185995222.

    MLA Style (8th edition)

  • MA, Miss, CI. "THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS." Master's thesis, University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1185995222

    Chicago Manual of Style (17th edition)

ucin1185995222
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© 2007, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.