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ucin1186764947.pdf (3.09 MB)
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DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS
Author Info
BANGE, ADAM F
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947
Abstract Details
Year and Degree
2007, PhD, University of Cincinnati, Arts and Sciences : Chemistry.
Abstract
The objective of this research was to improve the capabilities of immunosensing devices through miniaturization and using nanoscaled materials. Collaboration between the Chemical Sensors Group and the UC Department of Engineering was established to develop sensors and sensing systems using modern microfabrication technology. One facet of this collaboration included work to miniaturize a microbead-based sandwich immunoassay that was developed by previous group members. A procedure to fabricate poly(dimethylsiloxane) microfluidic devices was developed to control the movement of small amounts of immunoassay reagents and microbeads. The devices were then used to evaluate bead and fluid manipulation, and detection. While the electrochemical and fluorescence measurements required for an immunoassay were demonstrated, we were not able to efficiently manipulate microbeads due to their adsorption to the microchannel walls. Research was then done to improve the detection step of a miniaturized immunoassay. A microbead based sandwich immunoassay was developed using an interdigitated array (IDA) electrode with nanoscale dimensions (220 nm electrode width, 620 nm gap). The IDA was fabricated using an electron beam lithographic lift-off technique. After an antibody-assisted capture of antigen using paramagnetic microbeads, a β-galactosidase labeled secondary antibody was used to convert p-aminophenyl galactopyranoside (PAPG) into the redox active p-aminophenol (PAP). Amperometric detection of PAP with IDA electrodes at +300 and -200 mV vs. a Ag/AgCl reference electrode was used to measure the result, detecting MS2 bacteriophage concentrations as low as 10 ng/mL. The third research project focused on making a label-free immunosensor using an array of carbon nanotubes as an electrode and electrochemical impedance spectroscopy (EIS) for detection. Highly aligned multi-walled carbon nanotubes were grown by chemical vapor deposition using a metallic catalyst, Fe/Al2O3/SiO2, on Si wafers. The nanotube towers were removed from the silicon and cast in epoxy, then polished so that one end was exposed for electrical connection and the other used as the electrode array surface. The nanotubes were functionalized electrochemically to form carboxyl groups and then chemically conjugated to antibodies. EIS was used to directly monitor the antibody-antigen binding.
Committee
Dr. H Halsall (Advisor)
Pages
137 p.
Keywords
immunoassay
;
electrochemistry
;
Interdigitated array
;
microfluidic
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Citations
BANGE, A. F. (2007).
DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS
[Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947
APA Style (7th edition)
BANGE, ADAM.
DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS.
2007. University of Cincinnati, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947.
MLA Style (8th edition)
BANGE, ADAM. "DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS." Doctoral dissertation, University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947
Chicago Manual of Style (17th edition)
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Document number:
ucin1186764947
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Copyright Info
© 2007, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.