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Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) Test Kits for the Quantitative Determination of Endocrine Disrupting Compounds (EDCs) in Aqueous Phase Environmental Samples

Kleiner, Eric J.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282056078

Abstract Details

2010, MS, University of Cincinnati, Engineering and Applied Science: Environmental Engineering.
There is a glaring need to be able to measure for Endocrine Disrupting Compounds (EDCs) in aqueous environmental samples rapidly, inexpensively, and reliably. Commercially available enzyme-linked immunosorbent assay (ELISA) kits are a possible option but there is uncertainty on how reliable they can be in environmental matrices. In this study, four commercially available ELISA kits, E2 96 well, E2 magnetic particle, EE2 96 well, and AP 96 well were used to measure for E2, EE2, and NP. Samples were analyzed on each kit both directly, after only glass fiber filter cleanup, and after solid phase extraction (SPE). The samples were also analyzed by GCMS for the same compounds. The study was designed in a round robin fashion with a total of five of each kit split between three laboratories to analyze the same spiked sample. The study was done in four phases, with each phase a more complex matrix, beginning with de-ionized water (DI), then stream water, then wastewater effluent, then wastewater influent. The precision for each kit and GCMS method was measured by calculating the relative standard deviation (RSD) of triplicate sample analysis and the accuracy measured by calculating percent bias from the spiked known concentration of each sample. The RSD between the mean concentration results of each of the three laboratories was also calculated for each kit to measure for precision differences between labs. The RSD calculations between labs show that there was good precision between the labs for each kit. Overall the kits showed good precision with good accuracy results in de-ionized and stream water matrices and good precision with moderate accuracy results in wastewater matrix, depending on the kit. This compared well to the precision and accuracy results obtained by GCMS of the same samples, particularly in cleaner matrices. This would indicate that commercially available ELISA kits would make a valuable tool for measuring clean water and, at least, a semi-quantitative tool for screening complex matrices like wastewater for E2, EE2 and NP.
Makram Suidan, PhD (Committee Chair)
Margaret Kupferle, PhD, PE (Committee Member)
Marc Mills, PhD (Committee Member)
35 p.
Environmental Engineering
ELISA; Estrogen; Wastewater; Endocrine Disrupting Compounds; Estradiol; Nonylphenol

Recommended Citations

Citations

  • Kleiner, E. J. (2010). Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) Test Kits for the Quantitative Determination of Endocrine Disrupting Compounds (EDCs) in Aqueous Phase Environmental Samples [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282056078

    APA Style (7th edition)

  • Kleiner, Eric. Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) Test Kits for the Quantitative Determination of Endocrine Disrupting Compounds (EDCs) in Aqueous Phase Environmental Samples. 2010. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282056078.

    MLA Style (8th edition)

  • Kleiner, Eric. "Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) Test Kits for the Quantitative Determination of Endocrine Disrupting Compounds (EDCs) in Aqueous Phase Environmental Samples." Master's thesis, University of Cincinnati, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282056078

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Cincinnati and OhioLINK.