Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ucin1331296365.pdf (14.35 MB)

ETD Abstract Container

Abstract Header

The Role of Retromer in Regulating the Apical-Basal Polarity and the Immune Response during Drosophila Development

Zhou, Bo
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1331296365

Abstract Details

2012, PhD, University of Cincinnati, Medicine: Developmental Biology.
The retromer complex is a membrane anchored intracellular protein complex. Retromer recognizes specific transmembrane cargos and mediates their transportation from endosomes to trans-Golgi network (TGN). Vacuolar protein sorting-associated protein 35 (Vps35) is a major component of retromer. Through analyzing phenotypes of Drosophila vps35 (dvps35) mutants/RNAi, our lab previously identified a role of retromer in mediating WNT secretion. However, dvps35 mutants/RNAi displayed additional developmental defects which were not relevant to WNT secretion. This indicated additional roles of retromer during Drosophila development. My work presented here uncovered two additional roles of retromer in (1) regulating apical-basal polarity in epithelial cells; (2) suppressing auto-activation of the Drosophila immune system. Further analysis showed that retromer regulated apical-basal polarity through recycling Crumbs, which acted as a novel retromer cargo. I also found that retromer suppressed auto-activation of the immune system by regulating Spatzle, the ligand in the Toll signaling pathway. In summary, this dissertation work expanded our understanding of the transmembrane cargos and the biological functions of retromer.
Xinhua Lin, PhD (Committee Chair)
Jun Ma, PhD (Committee Member)
Dan Wiginton, PhD (Committee Member)
Robert Brackenbury, PhD (Committee Member)
Brian Gebelein, PhD (Committee Member)
113 p.
Biology
Retromer; apical-basal polarity; Crumbs; Toll pathway; vps35; Drosophila;

Recommended Citations

Citations

  • Zhou, B. (2012). The Role of Retromer in Regulating the Apical-Basal Polarity and the Immune Response during Drosophila Development [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1331296365

    APA Style (7th edition)

  • Zhou, Bo. The Role of Retromer in Regulating the Apical-Basal Polarity and the Immune Response during Drosophila Development. 2012. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1331296365.

    MLA Style (8th edition)

  • Zhou, Bo. "The Role of Retromer in Regulating the Apical-Basal Polarity and the Immune Response during Drosophila Development." Doctoral dissertation, University of Cincinnati, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1331296365

    Chicago Manual of Style (17th edition)

ucin1331296365
430
© 2012, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.