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Thermodynamics and Mass Transport of Biomolecule Adsorption onto Chromatographic Media

Desch, Rebecca J
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1382372858

Abstract Details

2013, PhD, University of Cincinnati, Engineering and Applied Science: Chemical Engineering.
Chromatography is governed by a complex and nuanced set of interactions among the adsorbate, adsorbent, and mobile phase components. Flow microcalorimetry (FMC) is the only technique to directly measure the enthalpy of adsorption in a packed bed; as such it is a valuable tool in improving understanding of the mechanisms underlying protein adsorption. Direct enthalpy measurements were paired with free energies of adsorption calculated from the equilibrium adsorption isotherms to determine adsorption entropy. The effects of varying characteristics of the stationary phase (pore size, composition, surface functionality) or mobile phase (temperature, ionic strength, pH, ethanol content) on adsorption energetics were evaluated.
Stephen Thiel, Ph.D. (Committee Chair)
Neville Degouvea-Pinto, Ph.D. (Committee Member)
Soon Jai Khang, Ph.D. (Committee Member)
Peter Panagiotis Smirniotis, Ph.D. (Committee Member)
Apryll Stalcup, Ph.D. (Committee Member)
382 p.
Chemical Engineering
Chromatography; Biomolecule Adsorption; Flow Microcalorimetry; Adsorption Thermodynamics; Adsorbent; Mobile phase

Recommended Citations

Citations

  • Desch, R. J. (2013). Thermodynamics and Mass Transport of Biomolecule Adsorption onto Chromatographic Media [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1382372858

    APA Style (7th edition)

  • Desch, Rebecca. Thermodynamics and Mass Transport of Biomolecule Adsorption onto Chromatographic Media. 2013. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1382372858.

    MLA Style (8th edition)

  • Desch, Rebecca. "Thermodynamics and Mass Transport of Biomolecule Adsorption onto Chromatographic Media." Doctoral dissertation, University of Cincinnati, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1382372858

    Chicago Manual of Style (17th edition)

ucin1382372858
238
© 2013, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.