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Crim1 Maintains Retinal Vascular Stability during Development by Regulating Endothelial Cell Vegfa Autocrine Signaling

Fan, Jieqing
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397734750

Abstract Details

2014, PhD, University of Cincinnati, Medicine: Molecular and Developmental Biology.
Angiogenesis defines the process in which new vessels grow from existing vessels. Revealing the molecular mechanisms involved in this process will help deepen our understanding of cancer, diabetes, and many other diseases, and will direct avenues for future development of pro-angiogenesis or anti-angiogenesis therapies. In this study, using murine retinal angiogenesis as a model system, we show that Crim1 (Cysteine-rich motor neuron 1), a type 1 trans-membrane protein, is highly expressed in angiogenic endothelial cells. Deletion of the Crim1 gene in endothelial cells causes defective retinal angiogenesis characterized by delayed vessel extension and reduced vessel density. We demonstrated that endothelial Crim1 prevents ectopic vessel regression. We showed that Vegfa autocrine signaling is required for angiogenesis and we provide in vivo and in vitro data consistent with a model in which Crim1 enhances the autocrine signaling activity of Vegfa in VECs in part via Vegfr2.
Richard Lang, Ph.D. (Committee Chair)
Michael Robinson, Ph.D. (Committee Member)
Vladimir Kalinichenko, M.D. Ph.D. (Committee Member)
Saulius Sumanas, Ph.D. (Committee Member)
James Wells, Ph.D. (Committee Member)
97 p.
Developmental Biology
Angiogenesis; Crim1; Endothelial cells; Vegfa; Vessel stability

Recommended Citations

Citations

  • Fan, J. (2014). Crim1 Maintains Retinal Vascular Stability during Development by Regulating Endothelial Cell Vegfa Autocrine Signaling [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397734750

    APA Style (7th edition)

  • Fan, Jieqing. Crim1 Maintains Retinal Vascular Stability during Development by Regulating Endothelial Cell Vegfa Autocrine Signaling. 2014. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397734750.

    MLA Style (8th edition)

  • Fan, Jieqing. "Crim1 Maintains Retinal Vascular Stability during Development by Regulating Endothelial Cell Vegfa Autocrine Signaling." Doctoral dissertation, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397734750

    Chicago Manual of Style (17th edition)

ucin1397734750
746
© 2014, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.