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Thyroid hormone signaling in developmental regulation in Xenopus

Choi, Jinyoung
http://orcid.org/0000-0001-6226-2792
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447158174

Abstract Details

2015, PhD, University of Cincinnati, Arts and Sciences: Biological Sciences.
The critical role of thyroid hormone (TH) in vertebrate development has been well shown in amphibian metamorphosis. When TH level increases, TH target tissues and organs undergo drastic developmental changes that are time- and tissue-specific. However, it has not been fully understood how the tissues can determine the timing and rate of developmental changes during metamorphosis. In this dissertation, I investigate the role of TH signaling proteins and TH receptors that have been hypothesized to regulate developmental timing and tissue-responsivity in TH-dependent development by examining Xenopus. First, I show the role of TH signaling proteins (TH transporters and cytoplasmic TH binding proteins) in the regulation of the timing of TH-dependent development. I examined various tissue-specific expression patterns and relative expression levels of the signaling proteins at different developmental stages. Using in-vivo functional analysis, I examined the role of the over-expressed signaling proteins in the timing of tissue transformation. Second, I show the role of unliganded TH receptors in developmental regulation. Using TRa knockout Xenopus tropicalis, I show that unliganded TRs are critical to the delaying of developmental initiation through the repression of TH-response genes. Altered levels of tissue responsivity to TH in TRa knockout animals show that unliganded TRs also contribute to the rate of tissue transformation in the early developmental period. Third, I dissect the role of TRa in development and growth rates during metamorphosis and show its impact on the determination of developmental completion. By examining intestinal development in TRa knockout animals, I show the tissue-specific role of TRa in TH-dependent development.
Daniel Buchholz, Ph.D. (Committee Chair)
Yun-Bo Shi, Ph.D. (Committee Member)
Edwin Griff, Ph.D. (Committee Member)
Joshua Gross, Ph.D. (Committee Member)
Kenneth Petren, Ph.D. (Committee Member)
158 p.
Biology
Thyroid hormone; Development; Thyroid hormone receptor; Thyroid hormone signaling proteins

Recommended Citations

Citations

  • Choi, J. (2015). Thyroid hormone signaling in developmental regulation in Xenopus [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447158174

    APA Style (7th edition)

  • Choi, Jinyoung. Thyroid hormone signaling in developmental regulation in Xenopus. 2015. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447158174.

    MLA Style (8th edition)

  • Choi, Jinyoung. "Thyroid hormone signaling in developmental regulation in Xenopus." Doctoral dissertation, University of Cincinnati, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447158174

    Chicago Manual of Style (17th edition)

ucin1447158174
296
© 2015, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.