Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


25777.pdf (5.26 MB)

ETD Abstract Container

Abstract Header

Bridging Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) from Metalloproteomics to the Undergraduate Curriculum

Donnell, Anna M
http://orcid.org/0000-0001-9188-4750
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504781774657886

Abstract Details

2017, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) has been a cornerstone of elemental analysis for the past three decades. The field of metallomics has emerged as the study of metals and their use for growth and survival within biological organisms.1-3 More recently, the field of metalloproteomics has harnessed the power of separation techniques coupled to metal detection for the study of metal transport, storage, and use by proteins, and this has expanded protein studies considerably.2-3 The process of lysing cells to analyze metalloproteins requires examination of current cell lysis techniques and the development of new methods in order to retain non-covalently bound metals and this remains a major challenge.4 In Chapter 2, various cell lysis techniques were investigated in an effort to analyze the metalloproteome of dimorphic fungus Histoplasma capsulatum, expanding knowledge of microbial metalloproteomes which are largely uncharacterized.5 Specifically, zinc metalloproteins were of greatest interest as zinc plays a critical role in immune response.6,7 The work of Chapter 2 was conducted with the assistance of three undergraduate researchers, Stephanie Lewis, Taylor Tocash, and Sami Abraham. As part of their undergraduate research experience, they developed materials for sample preparation, instrumental use, and data analysis for High Performance Liquid Chromatography (HPLC) and ICP-MS and they assisted in experimental design. These materials have been used by graduate students and undergraduates in research labs and at the undergraduate teaching lab at the University of Cincinnati. In addition, these materials provided the foundation for the development of the undergraduate laboratory experiment in Chapter 3 in which students determined the concentration of arsenic in commercial sinus wash and tap water using two modes of ICP-MS and compared the results to federal regulations. The addition of the ICP-MS experiment to the CHEM 3030L Instrumental Analysis Laboratory course was one component of a larger course redesign which included the revision and addition of laboratory experiments as well as efforts to address course learning outcomes and improve students’ laboratory report writing skills. A Scientific Reasoning Tool (SRT) was developed based on McNeill’s Claim Evidence Reasoning Model as a way to scaffold the process of organizing information before writing a laboratory report as described in Chapter 4.8 To determine the impact of the SRT on student laboratory reports, the ICP-MS experiment laboratory reports were independently analyzed, student surveys were administered, and instructor interviews were conducted. This work seeks to demonstrate how the use of ICP-MS in research laboratories can be accessible to undergraduate researchers as well as undergraduate students through the design of new experiments. The exposure to modern, complex instrumentation such as ICP-MS is not yet common at colleges and universities and presents an opportunity for integration in the curriculum to prepare students for careers in Science Technology Engineering and Math (STEM) fields.
Patrick Limbach, Ph.D. (Committee Chair)
Jonathan M Breiner, Ph.D. (Committee Member)
William Heineman, Ph.D. (Committee Member)
Kathleen Koenig, Ph.D. (Committee Member)
Anne Vonderheide, Ph.D. (Committee Member)
129 p.
Analytical Chemistry
ICP-MS; Histoplasma capsulatum; undergraduate research; instrumental laboratory; scientific argumentation; cell lysis

Recommended Citations

Citations

  • Donnell, A. M. (2017). Bridging Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) from Metalloproteomics to the Undergraduate Curriculum [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504781774657886

    APA Style (7th edition)

  • Donnell, Anna. Bridging Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) from Metalloproteomics to the Undergraduate Curriculum. 2017. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504781774657886.

    MLA Style (8th edition)

  • Donnell, Anna. "Bridging Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) from Metalloproteomics to the Undergraduate Curriculum." Doctoral dissertation, University of Cincinnati, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504781774657886

    Chicago Manual of Style (17th edition)

ucin1504781774657886
667
© 2017, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.