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The Stability of Lytic Sulfolobus Viruses

Gazi, Khaled S
http://orcid.org/0000-0003-2326-188X
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858582145599

Abstract Details

2017, MS, University of Cincinnati, Arts and Sciences: Biological Sciences.
Among the three domains of cellular life, archaea are the least understood, and functional information about archaeal viruses is very limited. For example, it is not known whether many of the viruses that infect hyperthermophilic archaea retain infectivity for long periods of time under the extreme conditions of geothermal environments. To investigate the capability of viruses to Infect under the extreme conditions of geothermal environments. A number of plaque-forming viruses related to Sulfolobus islandicus rod-shaped viruses (SIRVs), isolated from Yellowstone National Park in a previous study, were evaluated for stability under different stress conditions including high temperature, drying, and extremes of pH. Screening of 34 isolates revealed a 95-fold range of survival with respect to boiling for two hours and 94-fold range with respect to drying for 24 hours. Comparison of 10 viral strains chosen to represent the extremes of this range showed little correlation of stability with respect to different stresses. For example, three viral strains survived boiling but not drying. On the other hand, five strains that survived the drying stress did not survive the boiling temperature, whereas one strain survived both treatments and the last strain showed low survival of both. The basis for these differences has not been identified, but the extent of the variation suggests that multiple properties of each viral isolate combine to determine the biological stability of the virions. Finally, selection of stable viral particles from an unstable strain in regards to high temperature was possible.
Dennis Grogan, Ph.D. (Committee Chair)
Trinity Hamilton (Committee Member)
Brian Kinkle, Ph.D. (Committee Member)
93 p.
Microbiology
Hyperthermophilic archaea; Sulfolobus islandicus; SIRV; Virion inactivation; Selection; virus life-history trade-offs

Recommended Citations

Citations

  • Gazi, K. S. (2017). The Stability of Lytic Sulfolobus Viruses [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858582145599

    APA Style (7th edition)

  • Gazi, Khaled. The Stability of Lytic Sulfolobus Viruses. 2017. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858582145599.

    MLA Style (8th edition)

  • Gazi, Khaled. "The Stability of Lytic Sulfolobus Viruses." Master's thesis, University of Cincinnati, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858582145599

    Chicago Manual of Style (17th edition)

ucin1511858582145599
121
© 2017, some rights reserved.Creative Commons License The Stability of Lytic Sulfolobus Viruses by Khaled S Gazi is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.