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Understanding Surfactant Skin Irritation by Probing the Relationship between the Structure and the Function of Micelles

Ade-Browne, Chandra
http://orcid.org/0000-0002-8714-6007
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1523629545916627

Abstract Details

2018, MS, University of Cincinnati, Pharmacy: Pharmaceutical Sciences.
It is established that the cleansing properties and irritation potential of surfactants can be managed with the addition of other surfactants or polymers. However, the mechanism of the surfactant-induced irritation is not fully understood. In 2003, the Blankschtein group used 14C-radiolabelling assay and dynamic light scattering (DLS) to study the penetration of sodium dodecyl sulfate (SDS) and a system of SDS with polyethylene glycol (PEG) through porcine skin. They concluded that the micelles and monomers contributed to skin irritation and the irritation potential of a surfactant was dependent on the size of the assembly body (this is referred to as the “Blankschtein Hypothesis” within this body of work). Herein, we use combined small-angle neutron scattering (SANS), DLS, and proton nuclear magnetic resonance (1H-NMR) spectroscopy to investigate the shape, size, and solution dynamics of the surfactant systems to further elaborate the “Blankschtein Hypothesis”. The results revealed that shape and inter-micellar interactions, in addition to size, serve as important factors in determining surfactant skin penetration. The validity of the Blankschtein Hypothesis was furthered explored by adding a clinical component to the study. Part of Nicole McCardy’s MS thesis work was to determine if pre-clinical assays, including 14C-radiolabeled skin penetration assay and DLS, could be used to predict the clinical results observed with human subjects. A model was built to predict the harshness of mixed surfactant and surfactant-polymer composition on human skin, as measured by corneometry and visual dryness scores in a five-day forearm controlled application test (FCAT). A similar method was applied to another set of formulations, simplified to contain only one anionic surfactant. All of the surfactants in this set were structurally similar, differing only slightly in chain lengths and functional groups. The pre-clinical assays in this study were correlated with clinical results, as measured by corneometry, visual redness scores, visual dryness scores, and transepidermal water loss (TEWL) in a 1-day Mini-Immersion study. Although several attempts were made, the results did not allow a statistically significant model to be built. It is speculated that the catalog of structurally similar surfactants caused the results for various assays to be too narrow, precluding the development of a meaningful model. At the current status, the results from the mini-immersion study neither contradicts nor supports the Blankschtein Hypothesis.
Harshita Kumari, Ph.D. (Committee Chair)
Gerald Kasting, Ph.D. (Committee Member)
Michael Weaver, Ph.D. (Committee Member)
119 p.
Pharmaceuticals
surfactant; surfactant irritation; sodium lauryl sulfate; small-angle neutron scattering; dynamic light scattering

Recommended Citations

Citations

  • Ade-Browne, C. (2018). Understanding Surfactant Skin Irritation by Probing the Relationship between the Structure and the Function of Micelles [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1523629545916627

    APA Style (7th edition)

  • Ade-Browne, Chandra. Understanding Surfactant Skin Irritation by Probing the Relationship between the Structure and the Function of Micelles. 2018. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1523629545916627.

    MLA Style (8th edition)

  • Ade-Browne, Chandra. "Understanding Surfactant Skin Irritation by Probing the Relationship between the Structure and the Function of Micelles." Master's thesis, University of Cincinnati, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1523629545916627

    Chicago Manual of Style (17th edition)

ucin1523629545916627
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