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Magnesium metal implants and their effects on soft tissue repairs

An, Xiaoxian
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592395032696939

Abstract Details

2020, PhD, University of Cincinnati, Pharmacy: Pharmaceutical Sciences/Biopharmaceutics.
Peripheral nerve injuries, caused mainly by traumas, affect over 200,000 people yearly in the US. There is a great need to find an alternative to autografts, the current clinical standard treatment, for the injuries, especially the ones resulting in long gaps (> 3 cm). In this research, we investigated on applying Magnesium (Mg) metal filaments as physical guidance to direct nerve regenerations in long-gap defects. Our overall hypothesis is that with the combination of porous conduits, Mg filaments, with additional factors like CNTF or Mg2+ are a promising method in treating long-gap peripheral nerve injuries. On the basis of our previous results, we first proposed that a novel polycaprolactone (PCL) mesh electrospun with Mg metal particles will be a promising candidate as a conduit material. Multiple mechanical and biological features of the mesh were characterized, as well as in vivo tissue response after subcutaneous implantation. The data revealed the presence of Mg metal was beneficial in immune-modulation and tissue repair. One mesh type, PM10, has the desirable properties and was then made into conduits. We hypothesized the conduits made from PM10 would support the repair and later tested it in the sciatic injured rats with stranded Mg Resoloy® wire inserted inside the conduits. The behaviors of the animals were monitored for 14 weeks before collecting the nerve tissues for histological assessments. Though histology shown that the regenerated tissue was healthy, no equivalent functional recovery has been observed compared to isografts. MicroCT scanning revealed that the conduits swelled and blocked the inner space for tissue growth. Besides altering to a more suitable conduit material (polysulfone), we proposed adding a growth factor, with or without the presence of Mg2+ salt solution, will improve the repair outcomes. The behavioral data indicated that empty polysulfone conduits with/without CNTF gave the best repairs. Only behavioral results were included due to the time limited and we expect the future histology will reveals more detail about the effects of Mg. In general, this study shed lights on nerve regenerating effects of Mg and tissue response to Mg when using it in peripheral nerve repair.
Sarah Pixley, Ph.D. (Committee Chair)
Narayan Bhattarai, Ph.D. (Committee Member)
Gary Gudelsky, Ph.D. (Committee Member)
John MacLennan, Ph.D. (Committee Member)
Matthew Robson, Ph.D. (Committee Member)
Vesselin Shanov, Ph.D. (Committee Member)
Judith Strong, Ph.D. (Committee Member)
233 p.
Biomedical Research
Magnesium; Peripheral nerve repair; Conduit; Biomaterial

Recommended Citations

Citations

  • An, X. (2020). Magnesium metal implants and their effects on soft tissue repairs [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592395032696939

    APA Style (7th edition)

  • An, Xiaoxian. Magnesium metal implants and their effects on soft tissue repairs. 2020. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592395032696939.

    MLA Style (8th edition)

  • An, Xiaoxian. "Magnesium metal implants and their effects on soft tissue repairs." Doctoral dissertation, University of Cincinnati, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592395032696939

    Chicago Manual of Style (17th edition)

ucin1592395032696939
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This open access ETD is published by University of Cincinnati and OhioLINK.