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Sensing of Small Molecules, Biomarkers, and Pathogens using Unique Plasmonic Assay Platforms

Cary, ReJeana
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595848703283784

Abstract Details

2020, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
Noble metal nanoparticles have been used as sensors utilizing the phenomenon localized surface plasmon resonance (LSPR). LSPR based sensors are competitive because of the high sensitivity and can be made label-free. LSPR sensors have great potential as diagnostic tools for point-of-care and in-field scenarios. In this work, I have developed three unique sensors that utilizes LSPR. First, a nanoparticle-based localized surface plasmon resonance (LSPR) assay to detect copper ions in biological samples in vitro was developed. After reducing the cellular Cu2+ ions to Cu+ ions using ascorbic acid, a `click’ reaction is carried out to covalently couple a dye to the surface of a gold nanoparticle array. Then utilizing the ability to fabricate LSPR arrays on substrates we developed durable flexible nanoparticle substrates for point-of-care sensing. Second, I developed a rapid, LSPR based diagnostic test for chlamydia without the need for amplification. This technique can be developed into a strip and be used for other STIs in a multiplexed manner. Last, a flexible plastic, sensitive sensor for cortisol and neuropeptide y was developed. These sensors show low limits of detection, good reproducibility, and good selectivity in complex biological samples. Moreover, they all show excellent promise as point-of-care sensors which can greatly expand medical diagnostics for at-home and in the field use.
Laura Sagle, Ph.D. (Committee Chair)
Peng Zhang, Ph.D. (Committee Chair)
In-Kwon Kim, Ph.D. (Committee Member)
Pearl Tsang, Ph.D. (Committee Member)
76 p.
Analytical Chemistry
point-of-care diagnostics; localized surface plasmon resonance; nanoparticle arrays; Analytical chemistry; Rapid DNA diagnostics; Flexible plastic substrates

Recommended Citations

Citations

  • Cary, R. (2020). Sensing of Small Molecules, Biomarkers, and Pathogens using Unique Plasmonic Assay Platforms [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595848703283784

    APA Style (7th edition)

  • Cary, ReJeana. Sensing of Small Molecules, Biomarkers, and Pathogens using Unique Plasmonic Assay Platforms. 2020. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595848703283784.

    MLA Style (8th edition)

  • Cary, ReJeana. "Sensing of Small Molecules, Biomarkers, and Pathogens using Unique Plasmonic Assay Platforms." Doctoral dissertation, University of Cincinnati, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595848703283784

    Chicago Manual of Style (17th edition)

ucin1595848703283784
321
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This open access ETD is published by University of Cincinnati and OhioLINK.