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Analysis of clade structure and gene flow in Caenorhabditis briggsae

Hampton, Rachael M.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1165949567

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2006, Master of Science (MS), Wright State University, Biological Sciences.
Hampton, Rachael M. M.S., Department of Biological Sciences, Wright State University, 2006. Analysis of clade structure and gene flow in Caenorhabditis briggsae. In this study multilocus DNA sequence variation was examined in geographic isolates of C. briggsae to determine clade structure and to test for evidence of gene flow between clades. Sequence data were obtained for a total of seventeen genes scattered throughout the genome for five C. briggsae strains. Phylogenetic reconstructions at thirteen of seventeen loci strongly supported the presence of two separate clades with AF16 and VT847 comprising clade 1 and HK104, HK105, and PB800 comprising clade 2. The remaining four loci were not informative for generating this distinction due to a lack of polymorphism in DNA sequence. Comparisons were also extended to include eleven additional strains for nine of these loci. Clade structure was also strongly supported by phylogenetic reconstructions at seven of these loci. Clade 1 comprised strains AF16, VT847, PS1185, and PS1186. Clade 2 comprised strains HK104, HK105, PB800, PB826, PB857, PB858, PB859, JU279, JU348, JU383, JU405, JU439, and JU441.The lack of support for clade structure at Cb-her-1 and Cb-glp-1 was likely due to gene flow. At Cb-her-1 shared polymorphisms were observed indicating the presence of gene flow. Thus, it appears that outcrossing does occur to some extent between the C. briggsae clades. To assist in identifying the genes contributing to reduce fitness in AF16::HK104 F2 hybrids, sequenced loci were also tested for cosegregation with developmental delay through bulk segregant analysis. Multiple pools of delayed AF16::HK104 F2 hybrids were collected then genotyped at thirteen loci. Of those tested, eleven consistently showed random segregation of both alleles. Genes Cb-egl-5 and Cb-glp-1 showed nonrandom segregation and both loci were skewed towards AF16. This indicated that the region linked to Cb-egl-5 and Cb-glp-1 on chromosome three is associated with developmental delay in AF16::HK104 F2 hybrids.
Scott Baird (Advisor)
141 p.
Biology, Genetics

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Citations

  • Hampton, R. M. (2006). Analysis of clade structure and gene flow in Caenorhabditis briggsae [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1165949567

    APA Style (7th edition)

  • Hampton, Rachael. Analysis of clade structure and gene flow in Caenorhabditis briggsae. 2006. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1165949567.

    MLA Style (8th edition)

  • Hampton, Rachael. "Analysis of clade structure and gene flow in Caenorhabditis briggsae." Master's thesis, Wright State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=wright1165949567

    Chicago Manual of Style (17th edition)

wright1165949567
© 2006, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.