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Cloning and Expression of Aquaporin in the Antennal Gland of Crayfish, Procambarus clarkii

Gao, Yang
http://rave.ohiolink.edu/etdc/view?acc_num=wright1249498322

Abstract Details

2009, Master of Science (MS), Wright State University, Biological Sciences.
Aquaporins (AQPs) are responsible for transferring water and other small molecules across the cell membrane. Relatively little research has been conducted on AQPs in crustaceans. To study the AQPs in crayfish, I hypothesis that 1) AQP is expressed in the antennal gland (kidney analogue) of crayfish; 2) crayfish AQP will show greater expression in tissues (gill and antennal gland) associated with volume regulation; 3) AQP mRNA expression in antennal gland and gill will increase in premolt and decrease in postmolt compared with intermolt level. RT-PCR and Real-time PCR assay were applied to test the hypothesis. A partial AQP gene was cloned from the antennal gland. This AQP was broadly expressed in all tissues tested. However, there was no significant difference in AQP mRNA expression in antennal gland and gill at three molting stages. This study constitutes a landmark in understanding of AQP function and evolution in crayfish.
Michele Wheatly, PhD (Advisor)
Michele Wheatly, PhD (Committee Chair)
David Goldstein, PhD (Committee Member)
Carissa Krane, PhD (Committee Member)
69 p.
Biology
Crayfish; Aquaporin; Antennal Gland

Recommended Citations

Citations

  • Gao, Y. (2009). Cloning and Expression of Aquaporin in the Antennal Gland of Crayfish, Procambarus clarkii [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1249498322

    APA Style (7th edition)

  • Gao, Yang. Cloning and Expression of Aquaporin in the Antennal Gland of Crayfish, Procambarus clarkii. 2009. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1249498322.

    MLA Style (8th edition)

  • Gao, Yang. "Cloning and Expression of Aquaporin in the Antennal Gland of Crayfish, Procambarus clarkii." Master's thesis, Wright State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1249498322

    Chicago Manual of Style (17th edition)

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© 2009, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.