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The Influence of Temperature on Population Growth and Allergen Production in Cultured House Dust Mites – Dermatophagoides pteronyssinus and Dermatophagoides farinae

Yella, Lakshmi

Abstract Details

2009, Master of Science (MS), Wright State University, Biological Sciences.
Dermatophagoides pteronyssinus and Dermatophagoides farinae are commercially cultured to produce materials to make extracts for diagnostic tests and immunotherapy. There is some speculation as to how the culture conditions such as time of culture, temperature, relative humidity and food might influence the mite populations and subsequent production of allergens. The purpose of our investigation was to see how temperature influenced the population growth and allergen production of cultured house dust mites. D. pteronyssinus and D. farinae were cultured at 20°C and 25°C. Samples were collected at two week intervals for live mite count, life stage composition and to determine Group 1 and Group 2 allergen concentrations. We saw that temperature did have an influence and that both species showed higher population growth rates and produced higher concentrations of Group 1 and Group 2 allergens than at 25°C compared to 20°C.
Larry G. Arlian, PhD (Advisor)
Barbara Hull, PhD (Committee Member)
John O. Stireman, PhD (Committee Member)
David L. Goldstein, PhD (Other)
Joseph F. Thomas, Jr., PhD (Other)
144 p.

Recommended Citations

Citations

  • Yella, L. (2009). The Influence of Temperature on Population Growth and Allergen Production in Cultured House Dust Mites – Dermatophagoides pteronyssinus and Dermatophagoides farinae [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1260772254

    APA Style (7th edition)

  • Yella, Lakshmi. The Influence of Temperature on Population Growth and Allergen Production in Cultured House Dust Mites – Dermatophagoides pteronyssinus and Dermatophagoides farinae. 2009. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1260772254.

    MLA Style (8th edition)

  • Yella, Lakshmi. "The Influence of Temperature on Population Growth and Allergen Production in Cultured House Dust Mites – Dermatophagoides pteronyssinus and Dermatophagoides farinae." Master's thesis, Wright State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1260772254

    Chicago Manual of Style (17th edition)