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Nuclear Reorganization and Gene Expression During Muscle Cell Differentiation

Pitstick, Amy L.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1309997417

Abstract Details

2011, Master of Science (MS), Wright State University, Biological Sciences.
Cellular differentiation is a process regulated by environmental, intracellular and intercellular factors. Myogenesis is a differentiation program in mammalian myotome cells in which Pax3 activates myogenic transcription factors to convert myotome cells to committed myoblasts. Myoblasts differentiate to become fully differentiated, multi-nucleated myotubes (Tajbakhsh et al., 1997). C2C12 cells are a model system for myogenesis. We noted that Bcl2-associated transcription factor (Btf) is upregulated during myogenesis. We observed that C2C12 nuclei reorganize during myogenesis. We observed that myoblast nuclei have approximately 5-10 small nucleoli, while nuclei in myotubes have fewer and larger nucleoli. Nucleolar reorganization occurs independently of multinucleation. Also, the reorganization is not a result of serum withdrawal, as other cell lines fail to show reorganization when grown in low serum conditions. Immunofluorescent data indicates nuclear speckles reorganize during myogenesis. Our results indicate that a large-scale reorganization of nucleoli and nuclear speckles occurs during myogenesis.
Paula Bubulya, PhD (Advisor)
Mill Miller, PhD (Committee Member)
Scott Baird, PhD (Committee Member)
158 p.
Biology; Cellular Biology
Myogenesis; Nuclear Reorganization

Recommended Citations

Citations

  • Pitstick, A. L. (2011). Nuclear Reorganization and Gene Expression During Muscle Cell Differentiation [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1309997417

    APA Style (7th edition)

  • Pitstick, Amy. Nuclear Reorganization and Gene Expression During Muscle Cell Differentiation. 2011. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1309997417.

    MLA Style (8th edition)

  • Pitstick, Amy. "Nuclear Reorganization and Gene Expression During Muscle Cell Differentiation." Master's thesis, Wright State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=wright1309997417

    Chicago Manual of Style (17th edition)

wright1309997417
463
© 2011, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.