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wright1326846462.pdf (1.58 MB)
ETD Abstract Container
Abstract Header
Expressing human Orai3 in insect cells for pharmacological studies
Author Info
Bennett, Orville R.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright1326846462
Abstract Details
Year and Degree
2012, Master of Science (MS), Wright State University, Physiology and Neuroscience.
Abstract
The Orai3 protein forms a Ca2+ channel with a pharmacological profile distinct from its close relative, the store-operated Ca2+ channel Orai1. Though closely related to Orai1, the function of Orai3 in humans is still unclear. This study attempts to contribute to the body of knowledge by undertaking a pharmacological analysis of Orai3 in insect cells. We describe here the creation of a vector capable of expressing the mammalian Orai3 gene on an insect background. We demonstrate the ability to induce gene expression of Orai3 in these insect cells, and then assess the effectiveness of this system by characterizing the pharmacological properties with the drug 2-aminoethoxydiphenyl borate (2-APB). The results show that the current strategy used to express Orai3 will require additional refinement before the system can be considered generally useful for pharmacological studies, because expression of Orai3 may be affected by native proteins. The interference of expression seems confined to Orai Ca2+ channels, as mammalian STIM1 was also expressed and a response to 2-APB, albeit unexpected, was observed.
Committee
J. Ashot Kozak, PhD (Advisor)
Robert Putnam, PhD (Committee Member)
Adrian Corbett, PhD (Committee Member)
Thomas Brown, PhD (Committee Member)
Pages
66 p.
Subject Headings
Biology
;
Cellular Biology
;
Molecular Biology
;
Physiology
Keywords
Orai3
;
2-APB
;
SOCE
;
store-operated calcium entry
;
Orai
;
CRAC
;
insect
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Citations
Bennett, O. R. (2012).
Expressing human Orai3 in insect cells for pharmacological studies
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1326846462
APA Style (7th edition)
Bennett, Orville.
Expressing human Orai3 in insect cells for pharmacological studies.
2012. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright1326846462.
MLA Style (8th edition)
Bennett, Orville. "Expressing human Orai3 in insect cells for pharmacological studies." Master's thesis, Wright State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1326846462
Chicago Manual of Style (17th edition)
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Document number:
wright1326846462
Download Count:
553
Copyright Info
© 2012, some rights reserved.
Expressing human Orai3 in insect cells for pharmacological studies by Orville R. Bennett is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.