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Jaggers Thesis Paper 8.30.15.pdf (1.85 MB)
ETD Abstract Container
Abstract Header
Is Polyvinylidene diflouride (PVDF) film biocompatible in the Murine Cochlea?
Author Info
Jaggers, Robert Maxwell
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright1440944212
Abstract Details
Year and Degree
2015, Master of Science (MS), Wright State University, Anatomy.
Abstract
Polyvinylidene fluoride (PVDF) film, a biomaterial that is an integral part of a Totally Implantable Sustainable Hearing Aide (TISHA) device, was examined to determine its biocompatibility within the murine cochlea. The biofilm was implanted in the ear using a round window cochleostomy in 7-9 week old male C57BL/6 mice. Three test groups containing seven mice each were implanted with PVDF film. Each mouse received a sham surgery on the non-implanted cochlea. Three test groups were examined at 48 hours, 3 weeks, and 3 months. At the end of this time the mouse was euthanized, the tissue containing the cochlea was extracted, decalcified in cal-rite solution, processed through a gradient of alcohols and xylene and embedded in paraffin. Slides were made and stained with Hemotoxylin and Eosin.The degree of the Foreign Body Reaction (FBR) in the cochlea with the implanted biofilm was determined quantitatively and qualitatively using nonparametric and parametric tests. We found that quantitatively the amount of erythrocytes significantly decreased between the 48 hour and 3 week survival length groups (1-way ANOVA, P-value=.020). The amount of neutrophils significantly decreased between the 48 hour and 3 week (1-way ANOVA, P-value=0.22) and 48 hour and 3 month survival length groups (1-way ANOVA, P-value=0.28). Membrane formation around the implant and presence of foreign body giant cells (FGBCs) and fibrocytes were evident in the chronically implanted animals. Damage to inner and outer hair cells of the Organ of Corti was observed in both implanted and control cochlea. Angiogenesis, a normal phase of the healing process, was significant only in the 3 week survival group (Fisher’s exact test, P-value=.0002). Animals were assigned individual scores that were related to a certain intensity level of the FBR. There was a significant increase in the average score of the FBR between the 48 hour and 3 week survival length group (Kruskal-Wallis test, P-value=.030). The majority of animals were assigned the lowest intensity level, very mild, while 3 animals received a mild intensity rating. We determined that PVDF film was biocompatible based on the observation that acute responses of the FBR decreased after the initial time of implantation, chronic response of the FBR did not progress in intensity after the 3 week time point, the average rank of the FBR was the lowest score on our intensity scale, and the components of the FBR did not spread from the site of implantation into surrounding areas of the cochlea. PVDF film did not interrupt the healing process of the host such as the clotting mechanism or become extruded from the cochlea.
Committee
Robert Goldenberg, M.D. (Advisor)
Greg Boivin, D.V.M. (Committee Member)
Larry Ream, Ph.D. (Committee Member)
Pages
91 p.
Subject Headings
Anatomy and Physiology
;
Biology
;
Biomedical Research
Keywords
anatomy
;
physiology
;
biomedical research
;
biology
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Citations
Jaggers, R. M. (2015).
Is Polyvinylidene diflouride (PVDF) film biocompatible in the Murine Cochlea?
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1440944212
APA Style (7th edition)
Jaggers, Robert.
Is Polyvinylidene diflouride (PVDF) film biocompatible in the Murine Cochlea? .
2015. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright1440944212.
MLA Style (8th edition)
Jaggers, Robert. "Is Polyvinylidene diflouride (PVDF) film biocompatible in the Murine Cochlea? ." Master's thesis, Wright State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=wright1440944212
Chicago Manual of Style (17th edition)
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Document number:
wright1440944212
Download Count:
627
Copyright Info
© 2015, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.