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Thesis by Qinmao Ye 0726 .pdf (1.3 MB)

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Exosomes Released from Multiple Myeloma Cells Influence the Angiogenic Function of Endothelial Cells by Regulating MicroRNA-29b

Ye, Qinmao
http://rave.ohiolink.edu/etdc/view?acc_num=wright1532474227694747

Abstract Details

2018, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Multiple myeloma is a hematological malignancy characterized by clonal proliferation of plasma cells generally caused by chromosomal abnormalities. It occurs in the bone marrow, which is the microenvironment of multiple myeloma. Exosomes (EXs) are 30-100 nm membrane-derived micro-vesicles containing various of bioactive molecules, such as microRNAs, to mediate the cell-cell interaction. Numerous studies reported that exosomes play a significant role in tumor microenvironment. Angiogenesis has the important implication in tumor exacerbation to supply nutrients to promote the progression of cancer cells through endothelial cells (ECs). Some studies demonstrated that microRNA-29b (miR-29b) can suppress tumor development and inhibit angiogenesis. Therefore, in this study, we designed experiments to research the relationship between exosomes released from multiple myeloma, miR-29b and angiogenesis in ECs. Two types of multiple myeloma cells, OPM2 and RPMI-8226 cell lines, were treated with C6-Ceramide. Their released exosomes (MM-EXC6-Cer) were collected, which enriched in miR-29b. MM-EXC6-Cer were cocultured with human umbilical vein endothelial cells (HUVECs) to test the effect on angiogenic function of HUVEC. The results showed that the EC proliferation, the tube formation, migration and vascular endothelial growth factor A (VEGFA) expression were decreased in ECs. In addition, miR-29b inhibitor was used in ECs, and could decrease the level of the miR-29b in ECs. Exosomes released from multiple myeloma cells (MM-EX) were cocultured with ECs, which were treated with miR-29b inhibitor, to examine the effects on EC angiogenic function. We found that EC proliferation, VEGFA expression, migration and tube formation were promoted. This data demonstrated that miR-29b can negatively modulate the angiogenic function of ECs through exosomes secreted by multiple myeloma cells.
Yanfang Chen, M.D., Ph.D. (Advisor)
Ji Chen Bihl, M.D., Ph.D. (Committee Member)
Ravi P. Sahu, Ph.D. (Committee Member)
57 p.
Biology; Biomedical Research; Pharmacology
Exosomes; MicroRNA; Multiple Myeloma; Endothelial cells; Angiogenesis

Recommended Citations

Citations

  • Ye, Q. (2018). Exosomes Released from Multiple Myeloma Cells Influence the Angiogenic Function of Endothelial Cells by Regulating MicroRNA-29b [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1532474227694747

    APA Style (7th edition)

  • Ye, Qinmao. Exosomes Released from Multiple Myeloma Cells Influence the Angiogenic Function of Endothelial Cells by Regulating MicroRNA-29b . 2018. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1532474227694747.

    MLA Style (8th edition)

  • Ye, Qinmao. "Exosomes Released from Multiple Myeloma Cells Influence the Angiogenic Function of Endothelial Cells by Regulating MicroRNA-29b ." Master's thesis, Wright State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1532474227694747

    Chicago Manual of Style (17th edition)

wright1532474227694747
374
© 2018, some rights reserved.Creative Commons License Exosomes Released from Multiple Myeloma Cells Influence the Angiogenic Function of Endothelial Cells by Regulating MicroRNA-29b by Qinmao Ye is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.