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Oladayo Oyebanji Final Thesis.pdf (1.53 MB)

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Topical Photodynamic Therapy Generates Microvesicle Particles

Oyebanji, Oladayo Ayobami
http://orcid.org/0000-0001-5028-7474
http://rave.ohiolink.edu/etdc/view?acc_num=wright1591035785031408

Abstract Details

2020, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Photodynamic therapy (PDT) involves the use of light at an appropriate wavelength acting on a photosensitizing chemical to cause cell death via generation of reactive oxygen species. PDT has been useful in the management of skin conditions (like acne, psoriasis) and cancers like superficial skin, esophageal and non-small cell lung cancers. In addition to these therapeutic effects, previous murine studies from our group have demonstrated that topical PDT induces immunosuppression in vivo. Thus, topical PDT of skin can generate systemic effects through unknown mechanisms. Our group showed that PDT induces an immunosuppressive effect which occurs partly via Platelet-Activating Factor Receptor (PAFR) signaling. Of importance, PAFR signaling can generate Microvesicle particles (MVP). MVPs are small extracellular membrane-enclosed particles believed to mediate cell-to-cell communication via the transport of bioactive signaling substances. The present studies tested if PDT could generate MVP release. Our studies used in vitro, ex vivo (human skin explants) and in vivo (murine) models. PDT increased MVP release across the different cell lines tested in vitro as well as treatment of human skin explants ex vivo. Murine studies also revealed a significant increase in MVP levels in skin and blood following PDT treatment. We also found a limited role for PAFR in this PDT-generated MVP release. These studies reveal a consistent production of MVPs following PDT and thus, provide insights into a possible novel mechanism whereby PDT exerts systemic effects via the generation of MVPs.
Jeffrey B. Travers, M.D., Ph.D. (Advisor)
Ravi P. Sahu, Ph.D. (Committee Member)
Ji C. Bihl, M.D., Ph.D. (Committee Member)
74 p.
Biomedical Research; Medicine; Pharmacology
Photodynamic therapy; Microvesicle particles; Extracellular vesicles

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Citations

  • Oyebanji, O. A. (2020). Topical Photodynamic Therapy Generates Microvesicle Particles [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1591035785031408

    APA Style (7th edition)

  • Oyebanji, Oladayo. Topical Photodynamic Therapy Generates Microvesicle Particles. 2020. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1591035785031408.

    MLA Style (8th edition)

  • Oyebanji, Oladayo. "Topical Photodynamic Therapy Generates Microvesicle Particles." Master's thesis, Wright State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1591035785031408

    Chicago Manual of Style (17th edition)

wright1591035785031408
205
© 2020, some rights reserved.Creative Commons License Topical Photodynamic Therapy Generates Microvesicle Particles by Oladayo Ayobami Oyebanji is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.