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Organic Blue TADF Chromophore Tag For Monitoring Transfection Studies

Bresler, Brandon G.
http://rave.ohiolink.edu/etdc/view?acc_num=wright161038158466956

Abstract Details

2020, Master of Science (MS), Wright State University, Chemistry.
A novel organic TADF chromophore was synthesized via a 3-step process to emit a blue fluorescent light upon excitation. The chromophore was covalently attached to a 61% isobutyryl amidated poly(ethyleneimine) (PEI-IBAm0.61) non-viral gene vector utilized in gene transfection experiments. The gene vectors are loaded with a green fluorescent protein (GFP) and are monitored via fluorescence microscopy. The addition of the blue chromophore therefore allows for the monitoring of the movements of the PEI-IBAm0.61 through the full delivery mechanism of the GFP inside the cells. This can give vital information about the overall toxicity of these non-viral gene vectors to biological organisms upon release of the GFP by allowing for the observation of the ultimate fate of the polymer in a biological system.
Eric Fossum, Ph.D. (Advisor)
David Dolson, Ph.D. (Committee Member)
Ioana Pavel, Ph.D. (Committee Member)
75 p.
Chemistry
Organic TADF Chromophore; Blue TADF Chromophore; Thermally Activated Delayed Fluorescence; Gene Therapy; Transfection Studies; Polyethyleneimine

Recommended Citations

Citations

  • Bresler, B. G. (2020). Organic Blue TADF Chromophore Tag For Monitoring Transfection Studies [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright161038158466956

    APA Style (7th edition)

  • Bresler, Brandon. Organic Blue TADF Chromophore Tag For Monitoring Transfection Studies. 2020. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright161038158466956.

    MLA Style (8th edition)

  • Bresler, Brandon. "Organic Blue TADF Chromophore Tag For Monitoring Transfection Studies." Master's thesis, Wright State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright161038158466956

    Chicago Manual of Style (17th edition)

wright161038158466956
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This open access ETD is published by Wright State University and OhioLINK.