Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Extracellular vesicles from UVB irradiated keratinocytes contain cyclobutane pyrimidine dimers

Ginugu, Meghana Reddy
http://rave.ohiolink.edu/etdc/view?acc_num=wright1622305384538658

Abstract Details

2021, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Ultraviolet (UV) radiation induces the formation of cyclobutane pyrimidine dimers (CPDs) in genomic DNA, which are normally removed by nucleotide excision repair. However, the fate of these adducts remain largely unexplored. Detection of these photoproducts in body fluids could act as a predictor of UV exposure and enable a better understanding of the pathogenesis of photosensitive skin diseases, such as lupus. Using cultured human keratinocytes exposed to UVB radiation in vitro, ultracentrifugation of cell culture supernatants, and immunodot blot analysis of isolated DNA, we have found that a small fraction of CPDs is released from cells in a dose- and time-dependent manner in association with small extracellular vesicles (SEVs). Furthermore, pharmacological manipulation of cell signaling pathways revealed that caspase-dependent apoptotic signaling was critical to the release of SEVs containing CPDs. These results show for the first time that CPDs are released from UVB-irradiated cells and co-purify with SEVs.
Michael George Kemp, Ph.D. (Advisor)
Jeffrey B. Travers, M.D., Ph.D. (Committee Member)
Ji C. Bihl, M.D., Ph.D. (Committee Member)
Alex Carpenter, Post Doc (Other)
85 p.
Pharmaceuticals; Pharmacology; Pharmacy Sciences
UVB; irradiated keratinocytes; extracellular vesicles

Recommended Citations

Citations

  • Ginugu, M. R. (2021). Extracellular vesicles from UVB irradiated keratinocytes contain cyclobutane pyrimidine dimers [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1622305384538658

    APA Style (7th edition)

  • Ginugu, Meghana. Extracellular vesicles from UVB irradiated keratinocytes contain cyclobutane pyrimidine dimers. 2021. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1622305384538658.

    MLA Style (8th edition)

  • Ginugu, Meghana. "Extracellular vesicles from UVB irradiated keratinocytes contain cyclobutane pyrimidine dimers." Master's thesis, Wright State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1622305384538658

    Chicago Manual of Style (17th edition)

wright1622305384538658
40
© 2021, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.