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Final Thesis.pdf (2.43 MB)
ETD Abstract Container
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CytoViva Hyperspectral Imaging for Comparing the Uptake and Transformation of AgNPs and Ag+ in Mitochondria
Author Info
Steingass, Kristina
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright1630267087380931
Abstract Details
Year and Degree
2021, Master of Science (MS), Wright State University, Chemistry.
Abstract
Nanomaterials have attracted significant attention in the last decade, with applications in everyday products. Amongst all known nanomaterials in use, silver is the leading metal, present in 531 different types of products, owing to their unique optical, electrical, antimicrobial, and thermal properties. Though silver nanoparticles (AgNPs) come in contact regularly with the general population, there is little known about their toxicity mechanism due to limited techniques for thorough analysis. CytoViva Hyperspectral Imaging (HSI) shows potential for filling these gaps. In this study, borohydride-capped AgNPs with an approximate diameter of 10 nm were synthesized using the modified Creighton method and characterized by Raman spectroscopy, inductively coupled plasma optical emission spectroscopy (ICP-OES), ultraviolet-visible absorption spectroscopy (UV-Vis), and CytoViva HSI. Vero 76 cells were then incubated for 2 hr with 0.0, 0.1, and 3.0 mg L-1 Ag+ and 3.0 mg L-1 borohydride-capped AgNPs and compared to control Vero 76 cells via CytoViva HSI. The hyperspectral signatures were deconvoluted utilizing peak centers of cholesterol, cytochrome c, and phospholipids determined though a Voigt fit. Visually, the 3.0 mg L-1 Ag+ and AgNP exposures displayed cell degradation through the presence of cell debris and change in cell shape. Additionally, t-tests performed on the deconvoluted peaks showed statistical significance in Ag+ and AgNP groups, especially with the peak associated with cytochrome c, indicating mitochondrial interaction.
Committee
Ioana Pavel, Ph.D. (Advisor)
David Dolson, Ph.D. (Committee Member)
Steven Higgins, Ph.D. (Committee Member)
Pages
59 p.
Subject Headings
Chemistry
Keywords
nanomaterials
;
silver nanoparticles
;
AgNPs
;
CytoViva Hyperspectral Imaging
;
HSI
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Citations
Steingass, K. (2021).
CytoViva Hyperspectral Imaging for Comparing the Uptake and Transformation of AgNPs and Ag+ in Mitochondria
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1630267087380931
APA Style (7th edition)
Steingass, Kristina.
CytoViva Hyperspectral Imaging for Comparing the Uptake and Transformation of AgNPs and Ag+ in Mitochondria.
2021. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright1630267087380931.
MLA Style (8th edition)
Steingass, Kristina. "CytoViva Hyperspectral Imaging for Comparing the Uptake and Transformation of AgNPs and Ag+ in Mitochondria." Master's thesis, Wright State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1630267087380931
Chicago Manual of Style (17th edition)
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Document number:
wright1630267087380931
Download Count:
330
Copyright Info
© 2021, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.