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Sprague MSc Thesis Final V.2.pdf (1.77 MB)

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Reconstruction of gut microbiome via intermittent feeding

Sprague, Kourtney
http://orcid.org/0000-0001-5123-1384
http://rave.ohiolink.edu/etdc/view?acc_num=wright1661984504255085

Abstract Details

2022, Master of Science (MS), Wright State University, Biochemistry and Molecular Biology.
The benefits of intermittent fasting have been studied across many facets of health. It is known that physiologically fasting results in a metabolic switch from liver-derived glucose to adipose cell derived ketones to be used as energy and signaling molecules. Fasting down-regulates inflammation, increases expression of antioxidant defenses, and activates pathways for DNA repair and autophagy. While fasting or intermittent feeding effects on host physiology have been identified, the relationship between fasting and gut microbiome is not as well known. In times of gut rest, resident microbes undergo detoxication and motility, and in times of activity, gut microbes undergo DNA repair, energy metabolism, and cell growth. These daily fluctuations of microbial composition and function are mainly influenced by feeding patterns, and a change of these feeding patterns results in dysbiosis. In this study, we used an in vitro system to simulate the conditions of the human colon to evaluate gut microbiome fluctuations when switched to a once-per-day feeding pattern also known as intermittent feeding. This study found that intermittent feeding results in a significant increase in three beneficial genera (Mitsoukella, Bifidobacterium, Phoceaicola) and decrease in two detrimental genera (Gemmiger and Desulfovibrio) across all vessels. Cell density, community diversity, and short chain fatty acid production were all decreased by intermittent feeding. It appears that intermittent feeding has both beneficial and harmful effects on the human gut microbiota
Oleg Paliy, Ph.D. (Advisor)
Hongmei Ren, Ph.D. (Committee Member)
Michael Craig, Ph.D. (Committee Member)
59 p.
Microbiology
Intermittent feeding; Human gut microbiome; Bioreactor

Recommended Citations

Citations

  • Sprague, K. (2022). Reconstruction of gut microbiome via intermittent feeding [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1661984504255085

    APA Style (7th edition)

  • Sprague, Kourtney. Reconstruction of gut microbiome via intermittent feeding. 2022. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1661984504255085.

    MLA Style (8th edition)

  • Sprague, Kourtney. "Reconstruction of gut microbiome via intermittent feeding." Master's thesis, Wright State University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=wright1661984504255085

    Chicago Manual of Style (17th edition)

wright1661984504255085
57
© 2022, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.