Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

IFN-γ Increases the Expression of SARS-CoV-2 Receptors on Vero E6 cells

Madabattula, Bindu Madhavi
http://rave.ohiolink.edu/etdc/view?acc_num=wright1670953174642387

Abstract Details

2022, Master of Science (MS), Wright State University, Microbiology and Immunology.
Respiratory epithelial cells are the initial target for SARS-CoV-2 infection. IFN-γ is known to increase the expression of ACE-2, an initial receptor for SARS-CoV-2, on epithelial cells. This study focuses on examining the effect of IFN-γ for ACE-2, TMPRSS2, and neuropilin-1 on Vero E6 cells using two immunofluorescence methods, namely, direct (membrane) fluorescence method and Cytation5 method. Direct fluorescence was determined using an Accu-Scope and ImageJ analysis. Using this method, significance (p<0.023) was observed only for ACE-2 when Vero E6 cells were treated with IFN-γ. Cytation5 fluorescence was determined using a Bio-tek Cytation5 plate reader. The results showed that IFN-γ significantly increased (p<0.001) the expression of ACE-2, neuropilin-1, and TMPRSS2. These results indicate Cytation5 is a more sensitive method for determining the expression of receptors on Vero E6 cells. The elevated levels of SARS-CoV-2 receptors expression resulting from IFN-γ treatment makes the epithelial cells more susceptible targets of SARS-CoV-2 infection. IFN-γ is most likely provided by innate immune cells in the initial COVID-19 infection, consequently contributing to the severity of disease.
Nancy J. Bigley, Ph.D. (Committee Chair)
Dawn P. Wooley, Ph.D. (Committee Member)
Lucile E. Wrenshall, M.D., Ph.D. (Committee Member)
Marjorie M. Markopoulos, Ph.D. (Committee Member)
64 p.
Immunology; Microbiology
SARS-CoV-2 receptors; ACE-2 expression on Vero E6 Cells; ACE-2 and IFN-γ; COVID-19

Recommended Citations

Citations

  • Madabattula, B. M. (2022). IFN-γ Increases the Expression of SARS-CoV-2 Receptors on Vero E6 cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1670953174642387

    APA Style (7th edition)

  • Madabattula, Bindu Madhavi. IFN-γ Increases the Expression of SARS-CoV-2 Receptors on Vero E6 cells. 2022. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1670953174642387.

    MLA Style (8th edition)

  • Madabattula, Bindu Madhavi. "IFN-γ Increases the Expression of SARS-CoV-2 Receptors on Vero E6 cells." Master's thesis, Wright State University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=wright1670953174642387

    Chicago Manual of Style (17th edition)

wright1670953174642387
65
© 2022, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.