Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List

Full text release has been delayed at the author's request until June 01, 2024

ETD Abstract Container

Abstract Header

Deciphering the pathway of human astrovirus release from infected cells

Eduful, Joshua
http://rave.ohiolink.edu/etdc/view?acc_num=wright1685034003205798

Abstract Details

2023, Master of Science (MS), Wright State University, Biological Sciences.
During infection, viruses are released from infected cells and spread to neighboring cells either through a lytic pathway (by rupturing cells), a non-lytic pathway (such as the use of extracellular vesicles), or both pathways. Human astroviruses (HAstV) are highly prevalent non-enveloped positive sense RNA viruses. They are separated into three clades, classic-type, MLB-type and VA-type, in humans. They are identified as the third leading cause of viral gastroenteritis in children, the elderly, and immunocompromised patients. Yet, little is known about the replication, transmission, immunity, and epidemiology of these viruses. Thus, I sought to investigate the possible pathways HAstV utilize to exit infected cells. First, I investigated the role of cell lysis in HAstV release. To do this, I measured cell death in HAstV infected Caco2 and Huh7.5 cells WST-1 assay for cell proliferation, and Annexin V/propidium iodide staining for cell apoptosis using microscopy and flow cytometry. Interestingly, HAstV-1, MLB-2 and VA-1 did not show significant cytopathic effect in infected cells despite the several log increases in viral titer as determined by RT-qPCR. Second, we investigated the role of extracellular vesicles (EV) in HAstV release. Using pharmaceuticals to modulate EV synthesis, we found that HAstV release increased when Forskolin and Norepinephrine were used to enhance EV production in infected Caco2 and Huh7.5 cells. Conversely, inhibition of EV production by GW4869 significantly reduced HAstV release in infected cells. Additionally, EV isolated from infected cells support new infection without trypsin activation. Our data showed an association between EV produced by host cells and the released HAstV particles. This suggests that human astrovirus egress is non-lytic and may involve the use of EV
Abimbola O. Kolawole, Ph.D. (Advisor)
Paula Ann Bubulya, Ph.D. (Committee Member)
Shulin Ju, Ph.D. (Committee Member)
86 p.
Biology; Biomedical Research
Astrovirus; Extracellular vesicles

Recommended Citations

Citations

  • Eduful, J. (2023). Deciphering the pathway of human astrovirus release from infected cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1685034003205798

    APA Style (7th edition)

  • Eduful, Joshua. Deciphering the pathway of human astrovirus release from infected cells. 2023. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1685034003205798.

    MLA Style (8th edition)

  • Eduful, Joshua. "Deciphering the pathway of human astrovirus release from infected cells." Master's thesis, Wright State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=wright1685034003205798

    Chicago Manual of Style (17th edition)

wright1685034003205798
© 2023, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.