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Preparing and Cloning a Natural Killer Cell Hybridoma

Damico, Nicole

Abstract Details

2000, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
NK cells, a component of our innate immune system, are responsible for the elimination of virus-infected and tumor cells which bypass detection by T-cells. In these studies, we attempted to create a NK cell hybridoma clone, which would provide a testable pool of NK cells. Mouse splenocytes were fused with P3X mouse non-secreting myeloma cells in the presence of polyethylene glycol (PEG). Following HAT selection, the cells were tested for the presence of the mouse NK cell surface antigen DX5.
Diane Fagan (Advisor)
98 p.

Recommended Citations

Citations

  • Damico, N. (2000). Preparing and Cloning a Natural Killer Cell Hybridoma [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1004458025

    APA Style (7th edition)

  • Damico, Nicole. Preparing and Cloning a Natural Killer Cell Hybridoma. 2000. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1004458025.

    MLA Style (8th edition)

  • Damico, Nicole. "Preparing and Cloning a Natural Killer Cell Hybridoma." Master's thesis, Youngstown State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1004458025

    Chicago Manual of Style (17th edition)