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Applying Mesenchymal Stromal Cells and Platelet-Rich Plasma on a Collagen Matrix to Improve Fascial Repair

Perko, John C.
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1342115316

Abstract Details

2012, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Incisional hernias are areas of weakness in the tissue caused by a surgical wound that doesn’t heal completely. This study examines the contributions of mesenchymal stromal cells and platelet-rich plasma on wound repair by observing wound healing of the abdominal muscle in a rat model. A cell adhesion assay was performed to determine which mesh material MSCs bind to most effectively MSCs/PRP/mesh material were applied to a repaired surgical incision, after which, the rat recovered for 4 to 8 weeks. The abdominal muscle was then sectioned, paraffin embedded, and placed on slides to be stained with Mason’s trichrome. The thickness of collagen deposition was measured using Motic ImagesPlus 2.0. Collagen organization, abundance, and myocyte degeneration were also evaluated by a panel of 4 blind observers. Changes in the thickness of collagen deposition were not demonstrated, but neovascularization and collagen abundance in the muscle layer was significantly increased with the addition of MSC and PRP during wound healing. Increased collagen at the wound site may improve the overall tissue strength following wound repair.
Diana Fagan, PhD (Advisor)
Johanna Krontiris-Litowitz, PhD (Committee Member)
Mark Womble, PhD (Committee Member)
56 p.
Biology; Health Sciences; Immunology
Mesenchymal Stromal Cells (MSCs); Platelet-Rich Plasma (PRP); wound healing; connective tissue

Recommended Citations

Citations

  • Perko, J. C. (2012). Applying Mesenchymal Stromal Cells and Platelet-Rich Plasma on a Collagen Matrix to Improve Fascial Repair [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1342115316

    APA Style (7th edition)

  • Perko, John. Applying Mesenchymal Stromal Cells and Platelet-Rich Plasma on a Collagen Matrix to Improve Fascial Repair. 2012. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1342115316.

    MLA Style (8th edition)

  • Perko, John. "Applying Mesenchymal Stromal Cells and Platelet-Rich Plasma on a Collagen Matrix to Improve Fascial Repair." Master's thesis, Youngstown State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1342115316

    Chicago Manual of Style (17th edition)

ysu1342115316
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© 2012, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.