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Effects of temperature and anticoagulant on the in vitro quantitation of Leukocyre Expressed Mac-1 and Post-traumatic assay to predict the development of ARDS

Pitt, Tracy Shawn
http://rave.ohiolink.edu/etdc/view?acc_num=ysu996864777

Abstract Details

2001, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Blood from health volunteers was collected into tubes containing either EDTA, heparin or oxalate and incubated at room temperature for 0, 5, 10, 15, 30 and 60 minutes before being placed on ice (prevents in vitro stimulation). Subsequently, the leukocytes were indirectly labeled with fluorescence conjugated antibodies, isolated and then analyzed by flow cytometry. Based on the control study results is was determined that incubating blood samples at room temperature in EDTA, followed by placing the sample on ice with 15 minutes would produce minimal effects on Mac-1 expression in vitro. Therefore EDTA was chosen as the best anticoagulant for patient studies. Patient studies involved collecting blood from trauma patients in EDTA containing tubes at 0, 6, 12, 18, and 24 hours after initial trauma. The patient analysis utilized a protocol similar to the control studies. ARDS patients were found to show increased Mac-1 expression in EDTA anticoagulated blood when compared to non-ARDS patients. Therefore, these studies suggest that Mac-1 is differentially expressed in post-traumatic patients who will develop ARDS when compared to patients who do not develop ARDS.
Diana Fagan (Advisor)
118 p.
Adult Respiratory Distress Syndrome; ARDS; lungs

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Citations

  • Pitt, T. S. (2001). Effects of temperature and anticoagulant on the in vitro quantitation of Leukocyre Expressed Mac-1 and Post-traumatic assay to predict the development of ARDS [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu996864777

    APA Style (7th edition)

  • Pitt, Tracy. Effects of temperature and anticoagulant on the in vitro quantitation of Leukocyre Expressed Mac-1 and Post-traumatic assay to predict the development of ARDS. 2001. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu996864777.

    MLA Style (8th edition)

  • Pitt, Tracy. "Effects of temperature and anticoagulant on the in vitro quantitation of Leukocyre Expressed Mac-1 and Post-traumatic assay to predict the development of ARDS." Master's thesis, Youngstown State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ysu996864777

    Chicago Manual of Style (17th edition)

ysu996864777
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© 2001, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.